. Salaire De Naby Keita 2021, Abnormal T-cell function in B-cell chronic lymphocytic leukaemia While hundreds of antigens have been identified and have a unique CD number, only a small number of these are routinely used. National Library of Medicine The screening panel will be charged based on the number of markers tested (FIRST for first marker, ADD1 for each additional marker). Clinical features, laboratory findings, morphologic, cytogenetic features, and Epstein-Barr virus status were important factors for diagnosing aggressive NK cell leukemia. Atypical or abnormal cells can demonstrate . Flow cytometry immunophenotyping is used primarily to help diagnose and classify blood cell cancers (leukemias and lymphomas) and to help guide their treatment. What is Immunophenotyping? - News-Medical.net Rinsho Ketsueki. 1998 Feb;109(2):211-20. doi: 10.1093/ajcp/109.2.211. What is Immunophenotyping?. 1985 Oct;66(4):848-58 What does it mean I have a monoclonal B-cell lymphocytosis - PubMed As the number of abnormal cells increases in the bone marrow, they may crowd out and inhibit the production of normal white blood cells, red blood cells, and platelets, and eventually abnormal cells may also be released into the blood. no immunophenotypic abnormalities detected This technique helps in prognostication and is also used to differentiate between neoplastic and reactive expansions of lymphocytes. between patient and physician/doctor and the medical advice they may provide. MeSH terms Chromosome Aberrations Although the World Health Organization classification of AML takes into account immunophenotypic features, the criteria for the same in monocytic AML is not clearly defined. doi: 10.1371/journal.pone.0158827. While in other B-NHL subtypes, such as MZL and LPL, the light-chain restriction is the only abnormality detected by FC. For assistance, contact. A normal cell will display a pattern of antigens that correlates with the type and maturity of the cell. Atypical cells: Are they cancer? - Mayo Clinic TdT and PAX5 were performed in five of the seven patients with ABLB detected by FC. Jevremovic D, Olteanu H: Flow cytometry applications in the diagnosis of T/NK-cell lymphoproliferative disorders. Diverse Immunophenotypic Abnormalities in Adult Patients with Correlation of cytogenetic findings with clinical features in 18 patients with inv(3)(q21q26) or t(3;3)(q21;q26). By junio 4, 2022 masonry pilaster details junio 4, 2022 masonry pilaster details American Society for Clinical Pathology; 2007; Betters DM: Use of flow cytometry in clinical practice. A comparison of MBL with overt chronic lymphoproliferations revealed common aspects in the preclinical state, regarding both the kind of cytogenetic aberrations detected and . 7 In summary, blasts of AMoL can be. An original cytospin preparation (preferably unstained) must be included with the spinal fluid specimen so correlative morphologic evaluation can occur. News-Medical, viewed 04 March 2023, https://www.news-medical.net/health/What-is-Immunophenotyping.aspx. sharing sensitive information, make sure youre on a federal Leuk Res. Available online at https://www.lls.org/managing-your-cancer/lab-and-imaging-tests/blood-tests#Immunophenotyping. The results from your immunophenotyping are compared to the pattern of antigens for normal cells as well as to patterns that are associated with abnormal cells (e.g., cells present with leukemias and lymphomas). If no abnormalities are detected by the initial panel, no further flow cytometric assessment will be performed unless otherwise indicated by specific features of the clinical presentation or prior laboratory results. What is Immunophenotyping?. Tissue flow cytometry immunophenotyping in the diagnosis and no immunophenotypic abnormalities detected - bigbangblog.net 1. Upper endoscopy revealed a neoplastic growth at . . Grave Encounters What Happened To Kenny, Leuk Res. Label specimen as spinal . Available online at https://www.merckmanuals.com/professional/sec11/ch142/ch142b.html. JAMA Patient Page V301 (4) [On-line information]. Blood Adv. The immunophenotype of adult acute myeloid leukemia: high frequency of lymphoid antigen expression and comparison of immunophenotype, French-American-British classification, and karyotypic abnormalities. Maecker, H. et. Category filter: Show All (140)Most Common (2)Technology (21)Government & Military (34)Science & Medicine (22)Business (30)Organizations (68)Slang / Jargon (8) Acronym Definition NSA National Security Agency (US government) NSA Naval Support Activity NSA National Speakers Association NSA No Strings Attached NSA Naczelny Sad Administracyjny (Polish . Classification of MDS patients according to the patterns of expression of multiple. 88184-Flow cytometry; first cell surface, cytoplasmic or nuclear marker x 1, 88185-Flow cytometry; additional cell surface, cytoplasmic or nuclear marker (each), 88187-Flow Cytometry Interpretation, 2 to 8 Markers (if appropriate), 88188-Flow Cytometry Interpretation, 9 to 15 Markers (if appropriate), 88189-Flow Cytometry Interpretation, 16 or More Markers (if appropriate), Normal Reports | I just had a colposcopy done to follow up on an ASCUS pap with high risk HPV. Front Immunol. (Revised 2012). Abnormal Reports, SI Normal Reports | Cuneo A, Ferrant A, Michaux JL, Boogaerts M, Demuynck H, Bosly A, Doyen C, Carli MG, Piva N, Castoldi G, et al. Bone marrow immunophenotyping by flow cytometry in refractory cytopenia Learn more about how plasma cell neoplasms are diagnosed and treated in this expert-reviewed summary. Therefore, the need to explore a new marker that can . 2. Compilation of the top interviews, articles, and news in the last year. If not ordering electronically, complete, print, and send 1 of the following forms with the specimen: -Hematopathology/Cytogenetics Test Request (T726). Application of these criteria to a series of nearly 500 cases of lymphoma indicated that over 90% of B-lineage and about 80% of T-lineage neoplasms manifested immunophenotypic abnormalities that could distinguish them from benign, reactive lymphoid processes. Pp 244-247. The blood of an older child or adult normally contains some mature B cells, but circulating immature B cells are not normally present. In the present study, we describe both quantitative and qualitative immunophenotypic abnormalities involving BM B-cells in MDS patients. Available online at https://www.questdiagnostics.com/hcp/intguide/jsp/showintguidepage.jsp?fn=TG_Lymphoid_Neoplasms.htm. The .gov means its official. Leuk Lymphoma. National Cancer Institute [On-line information]. Submission of bilateral specimens is not required. Case presentation We report the case of a 64-year-old woman with gastric primary myeloid sarcoma with monocytic differentiatio. The granulocytes (67% of the total white blood cells) and monocytes (5% of the total white blood cells) reveal no significant immunophenotypic abnormalities. This form enables patients to ask specific questions about lab tests. 9. The volume of fluid necessary to phenotype the lymphocytes or blasts in spinal fluid depends upon the cell count in the specimen. Significant associations between immunophenotypic and karyotypic features were observed both within individual FAB subgroups and independently from morphological criteria. It may be used in follow up to a complete blood count (CBC) and WBC differential that show an increased number of lymphocytes or the presence of immature blood cells or other abnormal cell counts. Before Accessed April 2011. Genomic and immunophenotypic landscape of aggressive NK-cell leukemia. Rahul E, Ningombam A, Acharya S, Tanwar P, Ranjan A, Chopra A. Available online at https://emedicine.medscape.com/article/207631-overview. The objective of the present study was to assess whether a Compass database-guided analysis can be used to . NCCN Clinical Practice Guidelines in Oncology. Furthermore, abnormal T-cell populations can be detected by using a panel of antibodies; . The prognostic value of immunophenotyping in AML is controversial [ 3]. Accessibility The markers (antigens) that are present on the cells as detected by flow cytometry immunophenotyping will help characterize the cells present. If not ordering electronically, complete, print, and send 1 of the following forms with the specimen: Specimens will be initially triaged to determine which, if any, of. Specimen Stability Information: Refrigerated < or =96 hours. For spinal fluid specimens: spinal fluid cell and differential counts are required. Immunophenotyping, a common application in flow cytometry, allows multiple cell surface markers to be simultaneously characterized on a per-cell basis.Immunophenotyping can be difficult by flow cytometry, however, when only a small number of cells are available. -, N Engl J Med. Phenotypic analysis by flow cytometry of surface immunoglobulin light chains and B and T cell antigens in lymph nodes involved with non-Hodgkin's lymphoma. The volume of fluid necessary to phenotype the lymphocytes or blasts in serous effusions depends upon the cell count in the specimen. Copyright 2014 Mosby, Inc. All rights reserved. No immunophenotypic myeloid abnormalities were detected in the healthy donor bone marrow aspirates or in the 10 remission bone marrow aspirates from patients with a history of nonmyeloid neoplasia (Table 3). Cytometry B Clin Cytom. The immunophenotype of different immature, myeloid and B-cell lineage no immunophenotypic abnormalities detected - tecnogin.com By Samuel Pirruccello. -TCL-1 break-apart at 14q32, to exclude T-cell prolymphocytic leukemia in cases with CD4-positive T-cell lymphoproliferative disorder (phenotypic aberrancy or very tight CD4+ population with high CD4:CD8 ratio). Your questions will be answered by a laboratory scientist as part of a voluntary service provided by one of our partners, American Society for Clinical Laboratory Science. In case 14, a patient had PCM with del(13q/RB1) as a sole abnormality detected by FISH and this patient's disease remained active during the following 17 months. These abnormalities were related to immunophenotypic markers as detected using a consensual panel of monoclonal antibodies allowing lineage assignment and investigation of myeloid marker expression on blast cells. Flow cytometry is generally used as follow up testing after a complete blood count (CBC) or white blood cells scan . Ann Hematol. Immunophenotyping detects the presence or absence of antigens found on the surface or interior of blood cells. Specific groupings of these antigens are normally present on or within WBCs and are unique to specific cell types and stages of cell maturation. no immunophenotypic abnormalities detected [On-line information]. Viability 7AAD: 99%. Cancers (Basel). Craig, F. and Foon, K. (2008 April 15). J Adv Pract Oncol. Anaplastic lymphoma kinase protein was detected in about 33% (3/9) of ALCLs examined by flow cytometric immunophenotyping (FCI); expression was validated by immunohistochemical analysis. Or it can be the result of a specific treatment. CD56 (26.0%) and CD7 (20.8%) were the most commonly expressed lymphoid markers in AML patients. An ASCUS pap smear result is considered to be mildly abnormal. Accessed April 2011. Please enable it to take advantage of the complete set of features! Available online at https://www.cancer.gov/cancertopics/factsheet/detection/laboratory-tests. Immunophenotypic features of acute myeloid leukemia with inv(3)(q21q26.2)/t(3;3)(q21;q26.2). Abnormal immunophenotype provides a key diagnostic marker: a - PubMed PDF available for download at https://jama.ama-assn.org/content/301/4/452.full.pdf. (2019 January 3, Updated). No significant immunophenotypic abnormality was detected by flow cytometry. If no abnormalities are detected by the initial panel, no further flow cytometric assessment will be performed unless otherwise indicated by specific features of the clinical presentation or prior laboratory results. Specimen must arrive within 96 hours of collection. 2018 Oct;17(10):2226-2237. doi: 10.1158/1535-7163.MCT-18-0426. A total of 192 Chinese patients with acute myeloid leukemia (AML) were immunophenotyped by flow cytometry using a panel of monoclonal antibodies. Chronic lymphocytic leukemia is an extremely heterogeneous disease and prognostic factors such as chromosomal abnormalities are important predictors of time to first treatment and survival. Flow cytometric immunophenotyping is an established method for the detection of occult leptomeningeal disease in patients with aggressive B-cell non-Hodgkin lymphoma, and is increasingly being used in the evaluation of patients without an established diagnosis of lymphoma who present with signs and/or symptoms referable to the central nervous These tests may suggest lymphoma or leukemia, but more information is generally needed to confirm a diagnosis and to identify a specific type of leukemia or lymphoma. This test is appropriate for hematopoietic specimens only. For bone marrow testing, if cytogenetic tests are desired along with this test request, an additional specimen should be submitted. Exome sequencing analysis of gastric primary myeloid sarcoma with (2013 December 11). Hanson CA: Acute leukemias and myelodysplastic syndromes. government site. CD38 expression is not detected (<10%) No evidence of p53 (17p13) 4. No significant associations were detected between the presence of flow cytometric abnormalities (defined as 2 or more abnormalities) in RCC patients and age or sex, the presence of human leukocyte antigen (HLA)-DR15 (found in an increased frequency in adult low-grade MDS and aplastic anemia patients 33 32 and associated with a better response to Available online at https://emedicine.medscape.com/article/990113-overview. If abnormal cells are present in the bloodstream, a blood sample is often used for flow cytometry immunophenotyping as it is easy to obtain and less invasive than other collection methods. As mentioned, the immunophenotypic panels used evolved during the study, and not all antigens were studied in the entire MDS patient group . Smaller volumes can be used if there is a high cell count. CD34 cells can be detected in cord blood, bone marrow and in the peripheral blood of normal subjects, where they constitute respectively about 1.5% and 0.1-0.01% of the elements . 3. This technique involves immunostaining of smears of fluids from body cavities or aspirates of tissues. (Keren D, McCoy JP, Carey J: Flow Cytometry in Clinical Diagnosis. Available online through https://www.lls.org. and transmitted securely. Bookshelf A pathologist, often one specializing in the study of blood diseases and/or blood cell cancers (a hematopathologist), will consider the results from the complete blood count (CBC), differential, blood smear, bone marrow findings, and flow cytometry immunophenotyping as well as other tests in order to provide a diagnostic interpretation. Before The site is secure. Accessed April 2011. This technique also helps identify or confirm the cell of origin in non-hematopoietic neoplasia. Leuk Lymphoma. Mayo Clinic Mayo Medical Laboratories [On-line information]. If no abnormalities are detected by the initial panel, no further flow cytometric assessment will be performed unless otherwise indicated by specific features of the clinical presentation or prior laboratory results. The present results further confirm that IGH@ rearrangement is not a rare genomic abnormality in B-CLL, and also show both that t(14;19)(q32;q13.2) is the most common cytogenetic change involving IGH@ rearrangement detected by FISH in B-CLL and that IGH@ rearrangement is correlated with CD38 expression. Disclaimer. Do not aliquot. The lady explained that that meant I didn't have anything preconcerous, but she didn't see to know what it DID mean. Diagnostic Value of Flow Cytometry in Cases with Myelodysplasia. Originally, glass slides with fixed tissue sections were treated with an antibody that was specific for a type of antigen typically found on certain abnormal cells associated with a particular leukemia or lymphoma. Depending upon flow cytometry immunophenotyping results, a healthcare practitioner may determine how likely your cancer will respond to treatment and how aggressive the treatment might be. Type and frequency of immunophenotypic alterations detected on PB platelets from MDS patients (n = 44) versus normal control subjects (n=20). How Is Childhood Leukemia Diagnosed? 2010 May;34(5):594-7. doi: 10.1016/j.leukres.2009.08.029. The .gov means its official. 5. Pediatric Acute Lymphoblastic Leukemia. Several studies have identified a relationship between AML prognosis and antigens such as CD7, CD9, CD11b, CD13, CD14, CD15, CD33, CD34, and CD56, though some other studies report conflicting results. MeSH Shi M, Ternus JA, Ketterling RP, et al: Immunophenotypic and laboratory features of t(11;14)(q13;q32)-positive plasma cell neoplasms. Chronic active Epstein-Barr virus infection progresses to aggressive NK cell leukemia with a poor prognosis. Background Myeloid Sarcoma with monocytic differentiation is rare and quite likely is missed by surgical pathologists. A ONECARE MEDIA COMPANY. Immunophenotyping by Flow Cytometry - Testing.com no immunophenotypic abnormalities detected. eCollection 2016. Available online at https://www.mayomedicallaboratories.com/test-catalog/Overview/3287. Using a method of analysis relying solely on immunoarchitectural features of a given case, the authors were able to define immunologic criteria capable of differentiating benign from malignant lymphoid processes independent from conventional morphologic analysis. 2015 May;169(3):368-376. doi: 10.1111/bjh.13303, 5. 2020 May-Aug;24(2):195-199. doi: 10.4103/0973-029X.294653. . Leuk Lymphoma. Even normal aging can make cells appear abnormal. Please note that medical information found Am J Clin Pathol. In our case report, a middle-aged male . Overall, del(13q14) and +12 were the most common abnormalities (39%), whereas del(11q13), del(17p13), and del(6q23) were detected only in 3, 1, and 0 cases, respectively. An official website of the United States government. Would you like email updates of new search results? sharing sensitive information, make sure youre on a federal Flow cytometry immunophenotyping may be ordered when you have an increased number of lymphocytes (or sometimes an increase in another type of white blood cell, WBC), anemia, a decreased platelet count, or immature WBCs that are not normally seen in the blood. Quest Diagnostics [On-line information]. Available online at https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3409649/. If no abnormalities are detected by the initial panel, no further flow cytometric assessment will be performed unless otherwise indicated by specific features of the clinical presentation or prior laboratory results. More importantly, there are newer classes of treatment options like CAR-T therapy, bispecific T-cell engagers, and monoclonal antibodies thatselectively target molecules like CD19 or CD20. Leukemia/Lymphoma Immunophenotyping by Flow Cytometry. This site needs JavaScript to work properly. Multivariate analysis identified CD34 and CD9 expression as independently predictive of the presence of at least one cytogenetic abnormality (P < 10(-4) and P < 0.03, respectively). The course of treatment for your cancer will be determined by your health care practitioner and their team based on flow cytometry immunophenotyping and other tests that might be performed. Chen, Y. Immunophenotypic criteria for the diagnosis of non-Hodgkin's - PubMed Web: mayocliniclabs.com: Email: mcl@mayo.edu: Telephone: 800-533-1710: International: +1 855-379-3115: Values are valid only on day of printing For the individual abnormalities detected for each of the 27 immunophenotypic variables analyzed, a score was defined. Smaller volumes can be used if there is a high cell count. Immunophenotypic abnormalities of different B-NHL subtypes are overly heterogeneous; hence, including all markers in one screening tube with kappa and lambda is difficult. Accessed April 2011. These antigens are protein structures found on or within WBCs. Merck Manual for Healthcare Professionals [On-line information]. Imamura N, Kusunoki Y, Oda K, Abe K, Dohi H, Inada T, Kuramoto A, Kajihara H, Fujii H, Kawa K, et al. The percentage and pattern of cells staining for CD34, TdT, and PAX5 . There is a dim Kappa expression and dim CD20 expression. Lymphoid Neoplasms Laboratory Support of Diagnosis and Management Test Guide. This technique helps identify the lineage. Jevremovic D, Dronca RS, Morice WG, et al: CD5+ B-cell lymphoproliferative disorders: Beyond chronic lymphocytic leukemia and mantle cell lymphoma. Rereview of PB smears from these patients, who had typical cutaneous findings of MF, did not identify definitive Sezary cells. The triage panel is initially performed to evaluate for monotypic B cells by kappa and lambda light chain expression, increased numbers of blast cells by CD34 and CD45 expression along with side scatter gating, and increased plasma cells by CD45 expression and side scatter gating. Based on these findings, we provide an objective marker based on clinical data for the definite diagnosis of ANKL. If you have a leukemia or lymphoma, routine tests such as a complete blood count (CBC) and a WBC differentialmay show an increased number of white blood cells with a predominance of one type. Bethesda, MD 20894, Web Policies ARUP Consult. In univariate analysis, CD9, CD10, CD15, CD34 and TdT expression appeared significantly associated with chromosomal anomalies. If no abnormalities are detected by the initial panel, no further flow cytometric assessment will be performed unless otherwise indicated by specific features of the clinical presentation or prior laboratory results. Immunophenotypic, cytogenetic and clinical features of 192 AML patients Antibodies are made up of chains of protein : 2 long (heavy) chains and 2 shorter (light) chains. Accessed April 2011. Immunophenotyping has become extremely important not only in diagnosis and subclassification of AML but also in the detection of the minimal residual disease. The opinions expressed here are the views of the writer and do not necessarily reflect the views and opinions of News Medical. 2008 December 1; 112(12): 43844399. It can detect normal cells as well as abnormal cells whose pattern of markers are typically seen with specific types of leukemia and lymphoma. The immunophenotype of ANKL cells may differ from reactive NK cells in 4 respects. These may be the first indication of a possible blood cell cancer. Tests for Acute Lymphocytic Leukemia (ALL). Kruglov O, Johnson LDS, Minic A, Jordan K, Uger RA, Wong M, Sievers EL, Shou Y, Akilov OE. Accessed December 2014. Spectrum and trigger identification of hemophagocytic lymphohistiocytosis in adults: A single-center analysis of 555 cases. Maturation-associated immunophenotypic abnormalities in bone marrow Background: Atypical lymphocytosis is a common peripheral blood abnormality seen not only in Epstein-Barr virus (EBV)-associated acute infectious mononucleosis but also in other conditions, including other viral infections, cancer, immune . This site needs JavaScript to work properly. Unauthorized use of these marks is strictly prohibited. By continuing to browse this site you agree to our use of cookies. With the exception of the MB2 B-cell-associated antigen, no B- and T-cell differentiation antigen was detected in case 1. (PDF) Immunophenotypic Analysis of Anaplastic Large Cell - ResearchGate Verbal Irony In Romeo And Juliet Act 2. no immunophenotypic abnormalities detected FREE COVID TEST lansing school district spring break 2021 Book Appointment Now. Treatment of plasma cell neoplasms (including multiple myeloma, monoclonal gammopathy of undetermined significance, and plasmacytoma) includes observation, chemotherapy, radiation therapy, stem cell rescue, targeted therapy, immunotherapy, and supportive therapies. The main advantages of IHC are the possibility to correlate antigen expression with cell morphology and tissue architecture and the ability to detect a relatively low number of neoplastic cells, such as in Hodgkin's lymphoma (HL) or T-cell-rich large B-cell lymphoma (TCRBCL). while also discussing the various products Sartorius produces in order to aid in this. As the number of abnormal cells increase in a lymph node, the size of the lymph node increases. Federal government websites often end in .gov or .mil. In this case report of a child with mosaic T21 and DS-AMKL, flow cytometry performed on BMA showed no immunophenotypic abnormalities, morphological review of BMA revealed no clusters of tumor cells, and BMA failed to show the expected GATA1 mutation. In these cases, LSC analysis is a methodology of choice because of its low sample requirements. Additional FISH or molecular testing may be recommended by the Mayo pathologist to facilitate diagnosis. Flow lymphoma is used in the case of lymphoid neoplasms or when a lymphoid origin is suspected on the basis of cell morphology after staining. It can detect normal cells as well as abnormal cells whose pattern of markers are typically seen with specific types of leukemia and lymphoma. Evaluating lymphocytoses of undetermined etiology, Identifying B- and T-cell lymphoproliferative disorders involving blood and bone marrow Distinguishing acute lymphoblastic leukemia (ALL) from acute myeloid leukemia (AML) Immunologic subtyping of ALL Distinguishing reactive lymphocytes and lymphoid hyperplasia from malignant lymphoma Distinguishing between malignant lymphoma and acute leukemia Phenotypic subclassification of B- and T-cell chronic lymphoproliferative disorders, including chronic lymphocytic leukemia, mantle cell lymphoma, and hairy cell leukemia Recognizing AML with minimal morphologic or cytochemical evidence of differentiation Recognizing monoclonal plasma cells, Identifying B- and T-cell lymphoproliferative disorders involving blood and bone marrow, Distinguishing acute lymphoblastic leukemia (ALL) from acute myeloid leukemia (AML) Immunologic subtyping of ALL Distinguishing reactive lymphocytes and lymphoid hyperplasia from malignant lymphoma Distinguishing between malignant lymphoma and acute leukemia Phenotypic subclassification of B- and T-cell chronic lymphoproliferative disorders, including chronic lymphocytic leukemia, mantle cell lymphoma, and hairy cell leukemia Recognizing AML with minimal morphologic or cytochemical evidence of differentiation Recognizing monoclonal plasma cells, Distinguishing acute lymphoblastic leukemia (ALL) from acute myeloid leukemia (AML), Immunologic subtyping of ALL Distinguishing reactive lymphocytes and lymphoid hyperplasia from malignant lymphoma Distinguishing between malignant lymphoma and acute leukemia Phenotypic subclassification of B- and T-cell chronic lymphoproliferative disorders, including chronic lymphocytic leukemia, mantle cell lymphoma, and hairy cell leukemia Recognizing AML with minimal morphologic or cytochemical evidence of differentiation Recognizing monoclonal plasma cells, Distinguishing reactive lymphocytes and lymphoid hyperplasia from malignant lymphoma Distinguishing between malignant lymphoma and acute leukemia Phenotypic subclassification of B- and T-cell chronic lymphoproliferative disorders, including chronic lymphocytic leukemia, mantle cell lymphoma, and hairy cell leukemia Recognizing AML with minimal morphologic or cytochemical evidence of differentiation Recognizing monoclonal plasma cells, Distinguishing reactive lymphocytes and lymphoid hyperplasia from malignant lymphoma, Distinguishing between malignant lymphoma and acute leukemia Phenotypic subclassification of B- and T-cell chronic lymphoproliferative disorders, including chronic lymphocytic leukemia, mantle cell lymphoma, and hairy cell leukemia Recognizing AML with minimal morphologic or cytochemical evidence of differentiation Recognizing monoclonal plasma cells, Distinguishing between malignant lymphoma and acute leukemia, Phenotypic subclassification of B- and T-cell chronic lymphoproliferative disorders, including chronic lymphocytic leukemia, mantle cell lymphoma, and hairy cell leukemia Recognizing AML with minimal morphologic or cytochemical evidence of differentiation Recognizing monoclonal plasma cells, Phenotypic subclassification of B- and T-cell chronic lymphoproliferative disorders, including chronic lymphocytic leukemia, mantle cell lymphoma, and hairy cell leukemia, Recognizing AML with minimal morphologic or cytochemical evidence of differentiation Recognizing monoclonal plasma cells, Recognizing AML with minimal morphologic or cytochemical evidence of differentiation.